{"id":3178,"date":"2017-03-05T01:04:00","date_gmt":"2017-03-04T23:04:00","guid":{"rendered":"http:\/\/ddni.ro\/wps\/?page_id=3178"},"modified":"2019-09-14T01:33:47","modified_gmt":"2019-09-13T22:33:47","slug":"infrastructura","status":"publish","type":"page","link":"https:\/\/ddni.ro\/wps\/ro\/departamente\/laboratoare-si-baze-de-cercetare\/infrastructura\/","title":{"rendered":"Infrastructura"},"content":{"rendered":"

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image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/02\/transparent_logo.png” use_bg_overlay=”off” use_text_overlay=”off” _builder_version=”3.29.1″ header_font=”Roboto|on||on|” body_font=”||||” background_color=”#ffffff” background_image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2019\/09\/Lab_1_small.jpg” background_enable_image=”on” background_position=”center_right” button_icon_placement=”right” button_on_hover=”on” header_font_select=”Roboto” _i=”0″ _address=”1.0.0″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” 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background_position=”center_left” button_icon_placement=”right” button_on_hover=”on” header_font_select=”Roboto” _i=”1″ _address=”1.0.1″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_slide][et_pb_slide heading=”Vizibilitatea activit\u0103\u021bii de cercetare” button_text=”Articole Stiintifice” button_link=”https:\/\/ddni.ro\/wps\/ro\/departamente\/laboratoare-si-baze-de-cercetare\/vizibilitate\/#text” image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/02\/transparent_logo.png” use_bg_overlay=”off” use_text_overlay=”off” _builder_version=”3.29.1″ header_font=”Roboto|on||on|” body_font=”||||” background_color=”#ffffff” background_image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2019\/09\/Lab_2_small.jpg” background_enable_image=”on” background_position=”center_right” button_icon_placement=”right” button_on_hover=”on” header_font_select=”Roboto” _i=”2″ _address=”1.0.2″ button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” 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custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Roboto|on||on|” text_text_color=”#a0a0a0″ text_font_size=”28px” text_letter_spacing=”5px” header_font=”Open Sans|on||on|” header_font_size=”49px” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”center” module_alignment=”center” _i=”0″ _address=”4.0.0.0″]Laboratorul de Chimie<\/strong>
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”1″ _address=”4.1″ column_structure=”1_3,1_3,1_3″][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”4.1.0″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture1.png” alt=”Anton Paar, Multiwave 3000″ title_text=”Anton Paar, Multiwave 3000″ align=”center” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”4.1.0.0″][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”1″ _address=”4.1.1″ custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” _i=”0″ _address=”4.1.1.0″]\u00cen etapa de preg\u0103tire a probelor pentru determinarea concentra\u021biilor de metale grele, se realizeaz\u0103 mineralizarea acid\u0103 cu ajutorul cuptorului cu microunde Anton Paar, Multiwave 3000. Concentra\u021biile\u00a0de metale grele din probele analizate , sunt determinate cu ajutorul ICP-MS Elan DRC-e, cu aplicabilitate la determinarea concentra\u0163iilor mici a unui num\u0103r mare de elemente.\u00a0Elementele chimice care provin dintr-un lichid sunt nebulizate \u015fi aerosolii rezulta\u0163i sunt transporta\u0163i de argon \u00een tor\u0163a cu plasm\u0103. Ionii produ\u015fi sunt antrena\u0163i \u00een plasm\u0103 \u015fi introdu\u015fi, printr-o interfa\u0163\u0103, \u00eentr-un spectrometru de mas\u0103, conform standardului SR EN ISO 17294-2, 2005
\n[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”2″ _address=”4.1.2″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture2.png” alt=”ICP-MS Elan DRC-e” title_text=”ICP-MS Elan DRC-e” align=”center” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”4.1.2.0″][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”2″ _address=”4.2″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”4.2.0″ custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” _i=”0″ _address=”4.2.0.0″]<\/p>\n

Pentru extrac\u021bia micropoluan\u021bilor organici din probele de ap\u0103 de suprafa\u021b\u0103\/subteran\u0103 \u0219i uzat\u0103 se folose\u0219te sistemul automat de Extrac\u0163ia \u00een Faz\u0103 Solid\u0103 (Solid Phase Extractor), care asigur\u0103 un consum minim de solven\u021bi, timp de extrac\u021bie redus \u0219i de asemenea posibilitatea prelucr\u0103rii a 12 probe.<\/p>\n

Pentru probele solide flor\u0103, faun\u0103 sol\/sedimente se folose\u0219te sistemul automat Accelerated Solvent Extractor, ASE 300 DIONEX, care la presiune \u0219i temperatur\u0103 \u00eenalt\u0103, efectueaz\u0103 extrac\u021bii cu 4 solven\u021bi pentru 24 de probe simultan, reduc\u00e2ndu-se timpul necesar extrac\u021biilor.<\/p>\n

Analiza micropolua\u0163ilor organici se realizeaz\u0103 cu Gaz Cromatograful cu Detector cu Captur\u0103 de Electroni\u00a0Clarus 600 Perkin Elmer, a c\u0103rei configura\u021bie este pentru determinarea pesticidelor organoclorurate \u0219i compu\u0219ilor bifenilici policlorura\u021bi.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”3″ _address=”4.3″ column_structure=”1_4,1_4,1_4,1_4″][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”4.3.0″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture3.png” alt=”Sistem de Extrac\u021bie Rapid\u0103 cu Solvent” title_text=”Sistem de Extrac\u021bie Rapid\u0103 cu Solvent” align=”center” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”4.3.0.0″][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”1″ _address=”4.3.1″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture4.png” alt=”Sistemul de Extrac\u0163ia \u00een Faz\u0103 Solid\u0103” title_text=”Sistemul de Extrac\u0163ia \u00een Faz\u0103 Solid\u0103” align=”center” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”4.3.1.0″][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”2″ _address=”4.3.2″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture5.png” alt=”Rotoevaporator” title_text=”Rotoevaporator” align=”center” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”4.3.2.0″][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”3″ _address=”4.3.3″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture6.png” alt=”Gaz Cromatograf cu Detector cu Captur\u0103 de Electroni Clarus 600 Perkin Elmer” title_text=”Gaz Cromatograf cu Detector cu Captur\u0103 de Electroni Clarus 600 Perkin Elmer” align=”center” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”4.3.3.0″][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22″ bb_built=”1″ _i=”5″ _address=”5″][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”0″ _address=”5.0″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”5.0.0″ custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Roboto|on||on|” text_text_color=”#a0a0a0″ text_font_size=”28px” text_letter_spacing=”5px” header_font=”Open Sans|on||on|” header_font_size=”49px” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”center” module_alignment=”center” _i=”0″ _address=”5.0.0.0″]Laborator de Hidrobiologie<\/b><\/strong>
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”1″ _address=”5.1″ column_structure=”1_4,1_4,1_4,1_4″][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”5.1.0″ custom_padding__hover=”|||”][et_pb_blurb title=”Fluoroprobe ” image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/07\/Hidro_Inf_1.jpg” image_max_width=”none” _builder_version=”3.0.74″ body_font=”Roboto||||” text_orientation=”justified” _i=”0″ _address=”5.1.0.0″]<\/p>\n

Spectrofotometru submersibil pentru m\u0103sur\u0103tori \u201din situ\u201d a concentra\u021biilor de clorifil\u0103 \u201da\u201d, utilizat la determin\u0103ri cantitative a diferitelor clase de alge.<\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”1″ _address=”5.1.1″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/07\/Hidro_Inf_2.jpg” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”5.1.1.0″][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”2″ _address=”5.1.2″ custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Roboto|on|||” text_orientation=”justified” _i=”0″ _address=”5.1.2.0″]<\/p>\n

2 microscoape \u0219i un steromicroscop, pentru evaluari cantitative \u0219i calitative ale speciilor. Sunt utilizate la examinarea structurilor cu importan\u021b\u0103 taxonomic\u0103. Unul dintre microscoape este echipat cu un aparat foto digital \u0219i aplica\u021bie de procesare a imaginilor folositor la m\u0103sur\u0103tori morfometrice \u0219i la realizarea arhivei digitale a probelor analizate.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”3″ _address=”5.1.3″ custom_padding__hover=”|||”][et_pb_image src=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/07\/Hidro_Inf_3.jpg” align_tablet=”center” align_last_edited=”on|desktop” _builder_version=”3.23″ animation_style=”slide” animation_direction=”left” animation_duration=”500ms” animation_intensity_slide=”10%” _i=”0″ _address=”5.1.3.0″][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”3.22″ bb_built=”1″ _i=”6″ _address=”6″][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”0″ _address=”6.0″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”6.0.0″ custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Roboto|on||on|” text_text_color=”#a0a0a0″ text_font_size=”28px” text_letter_spacing=”5px” header_font=”Open Sans|on||on|” header_font_size=”49px” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”center” module_alignment=”center” _i=”0″ _address=”6.0.0.0″]<\/p>\n

LABORATOR DE GENETIC\u0102 \u0218I ECOLOGIE MOLECULAR\u0102<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”1″ _address=”6.1″][et_pb_column type=”4_4″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”6.1.0″ custom_padding__hover=”|||”][et_pb_text _builder_version=”3.27.4″ text_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” _i=”0″ _address=”6.1.0.0″]\u00cen concordan\u021b\u0103 cu abord\u0103rile diferitelor teme de cercetare \u0219i a protocoalelor utilizate, Laboratorul de Genetic\u0103 \u0219i Ecologie Molecular\u0103 dispune de o infrastructur\u0103 adecvat\u0103, la standarde europene. Echipamentele moderne din cadrul laboratorului permit: stocarea pe termen lung a probelor de ADN, izolarea \u0219i purificarea acizilor nucleici, amplificarea genic\u0103 a diferi\u021bilor markeri moleculari \u0219i analize bioinformatice utiliz\u00e2nd softuri de actualitate.
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”2″ _address=”6.2″ column_structure=”1_3,1_3,1_3″][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”6.2.0″ custom_padding__hover=”|||”][et_pb_blurb image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture7.png” image_max_width=”none” _builder_version=”3.0.74″ body_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” _i=”0″ _address=”6.2.0.0″]Ultra Congelator – 80<\/b><\/strong>\u00b0<\/b><\/strong>C<\/b><\/strong>Conservarea materialului genetic \u00een condi\u021bii optime (\u00eentre -60\u00b0C \u0219i-80 \u00b0C) pe termen lung ofer\u0103 un suportul \u0219tiin\u021bific \u0219i material pentru abordarea unor probleme \u0219tiin\u021bifice actuale \u0219i de asemenea permite ridicarea nivelului cercet\u0103rii din domeniul biologiei, ecologiei, biologiei moleculare, bioinformaticii \u0219i conservarea biodiversit\u0103\u021bii.
\n[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”1″ _address=”6.2.1″ custom_padding__hover=”|||”][et_pb_blurb image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture8.png” image_max_width=”320px” _builder_version=”3.0.74″ body_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” _i=”0″ _address=”6.2.1.0″]Hot\u0103 biologic\u0103 cu flux laminar <\/b><\/strong>Hota biologic\u0103 cu flux de aer laminar permite desf\u0103\u0219urarea etapelor de izolare ADN, elu\u021biea primerilor liofiliza\u021bi \u0219i realizarea mix-ului de reac\u021bie PCR \u00een condi\u021bii optime, evit\u00e2ndu-se riscul de contaminare al probelor \u0219i reactivilor stoc cu ADN exogen. Utilizarea unei hote biologice cu flux laminar elimin\u0103 suspiciunea unui rezultat fals pozitiv determinat de o poten\u021bial\u0103 contaminare a probelor \u0219i ofer\u0103 un grad de acurate\u021be al analizelor efectuate (la nivelul standardelor interna\u021bionale). De asemenea, echiparea hotei cu lamp\u0103 UV permite sterilizarea incintei de lucru dup\u0103 fiecare utilizare.
\n[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”2″ _address=”6.2.2″ custom_padding__hover=”|||”][et_pb_blurb image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture9.png” image_max_width=”300px” _builder_version=”3.0.74″ body_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” _i=”0″ _address=”6.2.2.0″]<\/p>\n

Izolarea \u0219i purificarea acizilor nucleici presupune o suit\u0103 de echipamente variate plec\u00e2nd de la centrifug\u0103 cu r\u0103cire, termoblock \u0219i spectrofotometru de tip nanodrop. Toate aceste aparate asigur\u0103 extrac\u021bia acizilor nucleici \u00een cantit\u0103\u021bi optime, cu un grad \u00eenalt de puritate.<\/p>\n

[\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row module_class=” et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”3″ _address=”6.3″ column_structure=”1_3,1_3,1_3″][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”0″ _address=”6.3.0″ custom_padding__hover=”|||”][et_pb_blurb image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture10.png” image_max_width=”280px” _builder_version=”3.0.74″ body_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” _i=”0″ _address=”6.3.0.0″]PCR thermalcycler<\/b><\/strong>\u00a0Aceste echipamente permit amplificarea genic\u0103 a markerilor moleculari de interes cu grad ridicat de repetabilitate. Thermocycler PCR cu gradient permite alegerea condi\u021biilor optime de reac\u021bie PCR (polymerase chain reaction) pentru un num\u0103r de p\u00e2n\u0103 la 96 probe in simultan, variind temperatura de hibridare \u00eentre 30 \u2013 99\u00b0C. Astfel, se scurteaz\u0103 foarte mult timpul alocat procesului de optimizare a reac\u0163iilor PCR prin faptul c\u0103 o singur\u0103 utilizare a acestui aparat, la o pereche de primeri, poate echivala cu minimum 8 runde diferite de amplificare \u00een aparatul PCR clasic.
\n[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”1″ _address=”6.3.1″ custom_padding__hover=”|||”][et_pb_blurb image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture12.png” image_max_width=”350px” _builder_version=”3.0.74″ body_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” _i=”0″ _address=”6.3.1.0″]Echipamente auxiliare:\u00a0<\/b><\/strong>Acestea asigur\u0103 buna func\u021bionare a laboratorului \u0219i ofer\u0103 suport pentru etapele premerg\u0103toare analizelor genetice. Echipamentele auxiliare din dotarea laboratorului sunt: Sistem de bidistilare a apei (ap\u0103 distilat\u0103 necesar\u0103 la prepararea diferitelor solu\u021bii), Autoclav orizontal (sterilizarea consumabilelor utilizate in etapele de analiz\u0103) \u0219i Cuptor cu microunde (prepararea gelurilor de agaroz\u0103).
\n[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”3.25″ custom_padding=”|||” _i=”2″ _address=”6.3.2″ custom_padding__hover=”|||”][et_pb_blurb image=”https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2017\/03\/Picture11.png” image_max_width=”none” _builder_version=”3.0.74″ body_font=”Roboto||||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”justified” custom_margin=”130px|||” _i=”0″ _address=”6.3.2.0″]Sisteme de electroforez\u0103 orizontal\u0103 <\/b><\/strong>Sistemele de electroforez\u0103 orizontal\u0103 permit migrarea produ\u0219ilor PCR \u00een gel de agaroz\u0103, etap\u0103 necesar\u0103 pentru estimarea lungimii fragmentelor amplificate \u0219i pentru verificarea calitativ\u0103 (prezen\u021ba sau absen\u021ba amplific\u0103rilor nespecifice \u0219i gradul de integritatea) a ampliconilor ob\u021binu\u021bi. Laboratorul de Genetic\u0103 \u0219i Ecologie Molecular\u0103 este echipat cu 3 sisteme de electroforez\u0103 orizontal\u0103 ce permite migrarea simultan\u0103 a unui num\u0103r mare de probe (~50).
\n[\/et_pb_blurb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ custom_padding_last_edited=”off|desktop” _builder_version=”3.27.4″ background_color=”#373d4b” overflow-x=”hidden” overflow-y=”hidden” custom_margin=”||0px||false|false” custom_padding=”44px|0px|61px|0px|false|false” global_module=”2802″ collapsed=”on” bb_built=”1″ _i=”10″ _address=”10″][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ module_class=” et_pb_row_fullwidth et_pb_row_fullwidth et_pb_row_fullwidth et_pb_row_fullwidth et_pb_row_fullwidth” _builder_version=”3.25″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”89%” width_tablet=”80%” width_last_edited=”on|desktop” max_width=”89%” max_width_tablet=”80%” max_width_last_edited=”on|desktop” make_fullwidth=”on” _i=”0″ _address=”7.0″][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||” _i=”0″ _address=”7.0.0″][et_pb_blurb title=”Institutul Na\u021bional de Cercetare – Dezvoltare %22Delta Dun\u0103rii%22″ icon_placement=”left” _builder_version=”3.0.90″ header_font=”Roboto|on||on|” header_font_size=”20px” body_font=”Roboto Condensed||on||” body_font_size=”20px” body_line_height=”1.1em” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”center” background_layout=”dark” _i=”0″ _address=”7.0.0.0″]<\/p>\n

Strada Babadag, nr. 165, CP. 820112, Tulcea, Romania<\/p>\n

[\/et_pb_blurb][et_pb_blurb title=”Zona membrilor” icon_placement=”left” _builder_version=”3.0.95″ header_font=”Roboto|on||on|” header_font_size=”20px” body_font=”Roboto Condensed||on||” body_font_size=”20px” body_line_height=”1.1em” background_size=”initial” background_position=”top_left” background_repeat=”repeat” text_orientation=”center” background_layout=”dark” _i=”1″ _address=”7.0.0.1″]<\/p>\n

Accesa\u021bi datele dumneavoastr\u0103 din intranet<\/p>\n

[\/et_pb_blurb][et_pb_button button_url=”http:\/\/192.168.13.253:8080\/cgi-bin\/” button_text=”LOGIN” _builder_version=”3.16″ custom_button=”on” button_text_color=”#ffffff” button_bg_color=”#008ab5″ button_border_color=”#008ab5″ button_border_radius=”0px” button_font=”|||on|||||” button_icon=”%%94%%” custom_margin=”|||15px” custom_padding=”|||” button_text_color_hover=”#008ab5″ button_border_color_hover=”#ffffff” button_bg_color_hover=”#ffffff” button_text_size__hover_enabled=”off” button_text_size__hover=”null” button_one_text_size__hover_enabled=”off” button_one_text_size__hover=”null” button_two_text_size__hover_enabled=”off” button_two_text_size__hover=”null” button_text_color__hover_enabled=”on” button_text_color__hover=”#008ab5″ button_one_text_color__hover_enabled=”off” button_one_text_color__hover=”null” button_two_text_color__hover_enabled=”off” button_two_text_color__hover=”null” button_border_width__hover_enabled=”off” button_border_width__hover=”null” button_one_border_width__hover_enabled=”off” button_one_border_width__hover=”null” button_two_border_width__hover_enabled=”off” button_two_border_width__hover=”null” button_border_color__hover_enabled=”on” button_border_color__hover=”#ffffff” button_one_border_color__hover_enabled=”off” button_one_border_color__hover=”null” button_two_border_color__hover_enabled=”off” button_two_border_color__hover=”null” button_border_radius__hover_enabled=”off” button_border_radius__hover=”null” button_one_border_radius__hover_enabled=”off” button_one_border_radius__hover=”null” button_two_border_radius__hover_enabled=”off” button_two_border_radius__hover=”null” button_letter_spacing__hover_enabled=”off” button_letter_spacing__hover=”null” button_one_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover=”null” button_two_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover=”null” button_bg_color__hover_enabled=”on” button_bg_color__hover=”#ffffff” button_one_bg_color__hover_enabled=”off” button_one_bg_color__hover=”null” button_two_bg_color__hover_enabled=”off” button_two_bg_color__hover=”null” _i=”2″ _address=”7.0.0.2″][\/et_pb_button][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||” _i=”1″ _address=”7.0.1″][et_pb_blurb title=”DEPARTAMENTE” _builder_version=”3.0.74″ header_font=”Roboto|on|||” header_font_size=”21px” body_font=”Roboto||on||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” background_layout=”dark” animation=”off” _i=”0″ _address=”7.0.1.0″]<\/p>\n

Conservarea Biodiversit\u0103\u021bii \u0219i Utilizarea Durabil\u0103 a Resurselor Naturale<\/a><\/p>\n

Restaurare Ecologic\u0103 \u0219i Redresare Specii<\/a><\/p>\n

Sistem Informa\u021bional \u0219i Geomatic\u0103<\/a><\/p>\n

Centrul pentru Studiul Bolilor Transfrontaliere, Emergente \u0219i Zoonozelor<\/a><\/p>\n

Laboratoare \u0219i Baze de Cercetare<\/a><\/p>\n

Centrul de Informare Tehnologic\u0103 “Delta Dun\u0103rii”<\/a><\/p>\n

Dezvoltare Tehnologic\u0103, Transfer Tehnologic \u0219i Planificare Spatial\u0103<\/a><\/p>\n

[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||” _i=”2″ _address=”7.0.2″][et_pb_blurb title=”DESPRE NOI” _builder_version=”3.0.105″ header_font=”Roboto|on|||” header_font_size=”21px” body_font=”Roboto||on||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” background_layout=”dark” custom_margin=”|||” animation=”off” _i=”0″ _address=”7.0.2.0″]<\/p>\n

Cercetare<\/a><\/p>\n

Management<\/a><\/p>\n

Administatie<\/a><\/p>\n

Centrul de Informare<\/a><\/p>\n

[\/et_pb_blurb][et_pb_code _i=”1″ _address=”7.0.2.1″][et_social_follow icon_style=”flip” icon_shape=”circle” icons_location=”top” col_number=”auto” counts=”true” counts_num=”30″ outer_color=”light” network_names=”true”][\/et_pb_code][et_pb_button _builder_version=”3.16″ button_text_size__hover_enabled=”off” button_text_size__hover=”null” button_one_text_size__hover_enabled=”off” button_one_text_size__hover=”null” button_two_text_size__hover_enabled=”off” button_two_text_size__hover=”null” button_text_color__hover_enabled=”off” button_text_color__hover=”null” button_one_text_color__hover_enabled=”off” button_one_text_color__hover=”null” button_two_text_color__hover_enabled=”off” button_two_text_color__hover=”null” button_border_width__hover_enabled=”off” button_border_width__hover=”null” button_one_border_width__hover_enabled=”off” button_one_border_width__hover=”null” button_two_border_width__hover_enabled=”off” button_two_border_width__hover=”null” button_border_color__hover_enabled=”off” button_border_color__hover=”null” button_one_border_color__hover_enabled=”off” button_one_border_color__hover=”null” button_two_border_color__hover_enabled=”off” button_two_border_color__hover=”null” button_border_radius__hover_enabled=”off” button_border_radius__hover=”null” button_one_border_radius__hover_enabled=”off” button_one_border_radius__hover=”null” button_two_border_radius__hover_enabled=”off” button_two_border_radius__hover=”null” button_letter_spacing__hover_enabled=”off” button_letter_spacing__hover=”null” button_one_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover=”null” button_two_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover=”null” button_bg_color__hover_enabled=”off” button_bg_color__hover=”null” button_one_bg_color__hover_enabled=”off” button_one_bg_color__hover=”null” button_two_bg_color__hover_enabled=”off” button_two_bg_color__hover=”null” _i=”2″ _address=”7.0.2.2″][\/et_pb_button][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”3.25″ custom_padding=”|||” custom_padding__hover=”|||” _i=”3″ _address=”7.0.3″][et_pb_blurb title=”RESURSE” _builder_version=”3.0.101″ header_font=”Roboto|on|||” header_font_size=”21px” body_font=”Roboto||on||” background_size=”initial” background_position=”top_left” background_repeat=”repeat” background_layout=”dark” animation=”off” _i=”0″ _address=”7.0.3.0″]<\/p>\n

Evenimente<\/a><\/p>\n

Analele Stiintifice ale Institutului<\/a><\/p>\n

Organigrama<\/a><\/p>\n

Consiliul de Administratie<\/a><\/p>\n

Strategia 2015 – 2020<\/a><\/p>\n

Achizitii Publice<\/a><\/p>\n

Platforma ERRIS<\/a><\/p>\n

[\/et_pb_blurb][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Laboratorul de Chimie \u00cen etapa de preg\u0103tire a probelor pentru determinarea concentra\u021biilor de metale grele, se realizeaz\u0103 mineralizarea acid\u0103 cu ajutorul cuptorului cu microunde Anton Paar, Multiwave 3000. Concentra\u021biile\u00a0de metale grele din probele analizate , sunt determinate cu ajutorul ICP-MS Elan DRC-e, cu aplicabilitate la determinarea concentra\u0163iilor mici a unui num\u0103r mare de elemente.\u00a0Elementele chimice […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":3162,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-3178","page","type-page","status-publish","hentry"],"yoast_head":"\nInfrastructura - Danube Delta National Institute for Research and Development<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ddni.ro\/wps\/ro\/departamente\/laboratoare-si-baze-de-cercetare\/infrastructura\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Infrastructura - Danube Delta National Institute for Research and Development\" \/>\n<meta property=\"og:description\" content=\"Laboratorul de Chimie \u00cen etapa de preg\u0103tire a probelor pentru determinarea concentra\u021biilor de metale grele, se realizeaz\u0103 mineralizarea acid\u0103 cu ajutorul cuptorului cu microunde Anton Paar, Multiwave 3000. Concentra\u021biile\u00a0de metale grele din probele analizate , sunt determinate cu ajutorul ICP-MS Elan DRC-e, cu aplicabilitate la determinarea concentra\u0163iilor mici a unui num\u0103r mare de elemente.\u00a0Elementele chimice […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ddni.ro\/wps\/ro\/departamente\/laboratoare-si-baze-de-cercetare\/infrastructura\/\" \/>\n<meta property=\"og:site_name\" content=\"Danube Delta National Institute for Research and Development\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/INCDDD\/\" \/>\n<meta property=\"article:modified_time\" content=\"2019-09-13T22:33:47+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ddni.ro\/wps\/wp-content\/uploads\/2020\/05\/INCDDD_4_3.png\" \/>\n\t<meta property=\"og:image:width\" content=\"416\" \/>\n\t<meta property=\"og:image:height\" content=\"331\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@ddni_ddtic\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"24 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/ddni.ro\\\/wps\\\/ro\\\/departamente\\\/laboratoare-si-baze-de-cercetare\\\/infrastructura\\\/\",\"url\":\"https:\\\/\\\/ddni.ro\\\/wps\\\/ro\\\/departamente\\\/laboratoare-si-baze-de-cercetare\\\/infrastructura\\\/\",\"name\":\"Infrastructura - 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